Impact of Larger Sputum Volume on XpertMTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis.

TitleImpact of Larger Sputum Volume on XpertMTB/RIF Assay Detection of Mycobacterium tuberculosis in Smear-Negative Individuals with Suspected Tuberculosis.
Publication TypeJournal Article
Year of Publication2017
AuthorsBadal-Faesen S, Firnhaber C, Kendall MA, Wu X, Grinsztejn B, Escada ROtavio da, Fernandez M, Hogg E, Sanne I, Johnson P, Alland D, Mazurek GH, Benator DA, Luetkemeyer AF
Corporate AuthorsAIDS Clinical Trials Group A5295, Tuberculosis Trials Consortium Study 34 Teams
JournalJ Clin Med
Volume6
Issue8
Date Published2017 Aug 07
ISSN2077-0383
Abstract

As a strategy to improve the sensitivity of nucleic acid-based testing in acid-fast bacilli (AFB) negative samples, larger volumes of sputum (5-10 mL) were tested with XpertMTB/RIF from 176 individuals with smear-negative sputum undergoing tuberculosis evaluation. Despite larger volumes, this strategy had a suboptimal sensitivity of 50% (4/8).

DOI10.3390/jcm6080078
Alternate JournalJ Clin Med
PubMed ID28783093
PubMed Central IDPMC5575580
Grant ListUM1 AI069476 / AI / NIAID NIH HHS / United States
UM1 AI069432 / AI / NIAID NIH HHS / United States
UM1 AI068634 / AI / NIAID NIH HHS / United States
U01 AI068636 / AI / NIAID NIH HHS / United States
UM1 AI069496 / AI / NIAID NIH HHS / United States
UM1 AI069463 / AI / NIAID NIH HHS / United States
UM1 AI106701 / AI / NIAID NIH HHS / United States
UM1 AI069419 / AI / NIAID NIH HHS / United States
UM1 AI068636 / AI / NIAID NIH HHS / United States