Integration of atazanavir into an existing liquid chromatography UV method for protease inhibitors: validation and application.

TitleIntegration of atazanavir into an existing liquid chromatography UV method for protease inhibitors: validation and application.
Publication TypeJournal Article
Year of Publication2007
AuthorsKeil K, Hochreitter J, DiFrancesco R, Zingman BS, Reichman RC, Fischl MA, Gripshover B, Morse GD
JournalTher Drug Monit
Volume29
Issue1
Pagination103-9
Date Published2007 Feb
ISSN0163-4356
KeywordsAtazanavir Sulfate, Calibration, Chromatography, High Pressure Liquid, Drug Monitoring, Drug Therapy, Combination, HIV Infections, HIV Protease Inhibitors, Humans, Lopinavir, Oligopeptides, Pyridines, Pyrimidinones, Quality Control, Reference Standards, Reproducibility of Results, Ritonavir, Spectrometry, Mass, Electrospray Ionization, Spectrophotometry, Ultraviolet, Time Factors
Abstract

Atazanavir (ATV) is a widely used human immunodeficiency virus (HIV)-1 protease inhibitor (PI) that, like other approved PIs, has been considered as a candidate for therapeutic drug monitoring (TDM). To provide ATV assay results that can be applied to patient management through TDM, the assay would need to perform in a manner consistent with Clinical Laboratory Improvement Amendments (CLIA) standards. To quantitate ATV concentrations in human plasma, the authors added ATV to a previously published reversed-phase high-performance liquid chromatography (HPLC) method from their laboratory. Detection was effected with use of a photodiode-array detector (PDA) collecting spectra at 248 nm. This method allows for detection of ATV to a lower limit of quantitation of 0.05 microg/mL, with an intra-assay coefficient of variation (CV%) of 8.9% or less over 5 days of testing and an interassay CV% ranging from 1.4 to 6.4%. The assay has met passing requirements for interlaboratory proficiency testing for 2 years nationally and internationally, with accuracy within +/-15% over all test samples. During 2 years, more than 100 batches of analyses have been performed and have proved the method is rugged, specific, and accurate. This assay method is currently used in the authors' clinical research program in TDM.

DOI10.1097/FTD.0b013e3180318ef3
Alternate JournalTher Drug Monit
PubMed ID17304157
Grant ListAI-51519 / AI / NIAID NIH HHS / United States
DA015024 / DA / NIDA NIH HHS / United States
U01-AI138858 / AI / NIAID NIH HHS / United States