Effect of low-dose IL-2 immunotherapy on frequency and phenotype of regulatory T cells and NK cells in HIV/HCV-coinfected patients.

TitleEffect of low-dose IL-2 immunotherapy on frequency and phenotype of regulatory T cells and NK cells in HIV/HCV-coinfected patients.
Publication TypeJournal Article
Year of Publication2008
AuthorsVelilla PA, Shata MT, Lages CS, Ying J, Fichtenbaum CJ, Chougnet C
JournalAIDS Res Hum Retroviruses
Volume24
Issue1
Pagination52-61
Date Published2008 Jan
ISSN0889-2229
KeywordsAdult, Antiretroviral Therapy, Highly Active, Antiviral Agents, Drug Therapy, Combination, Female, Hepacivirus, Hepatitis C, HIV Infections, HIV-1, Humans, Immunophenotyping, Immunotherapy, Interferons, Interleukin-2, Killer Cells, Natural, Male, Middle Aged, Polyethylene Glycols, Ribavirin, T-Lymphocytes, Regulatory, Treatment Outcome
Abstract

We evaluated the effect of low-dose IL-2 therapy (daily 1.2 MIU/m(2), subcutaneously) on the number and phenotype of regulatory T cells (T(regs)) and natural killer (NK) cells in HIV/HCV-coinfected patients taking antiretroviral therapy. The frequency and phenotype of circulating T(regs) (defined as CD3(+) CD4(+) CD25(high) or CD3(+) CD4(+) FOXP3(+)) and NK cells (CD3(-) CD16(+)/CD56(+)) were evaluated at baseline and after 12 weeks of treatment. The expression of CD25, CTLA-4, and granzymes A and B by CD4(+) FOXP3(+) cells, as well as the expression of KIR receptors (NKB1, CD158a, and NKAT2) on NK cells, was evaluated. Low doses of IL-2 resulted in the augmented frequency and absolute number of T(regs) in coinfected individuals. FOXP3 levels per cell as well as augmented CD25 and CTLA-4 expression by T(regs) suggested that IL-2 may lead to both expansion and activation of T(regs), although changes in the proportion of CD4(+) FOXP3(+) cells were not associated with changes in HCV viral load and CD4(+) cells between baseline and week 12. NK cell frequency also increased after IL-2 therapy. Interestingly, the pattern of expression of KIR receptors was changed by IL-2 treatment, since the frequency of NK cells expressing NKB1 augmented whereas the frequency of NK expressing CD158a and NKAT2 decreased.

DOI10.1089/aid.2007.0180
Alternate JournalAIDS Res. Hum. Retroviruses
PubMed ID18275348
Grant ListAI-25897 / AI / NIAID NIH HHS / United States
AI-38858 / AI / NIAID NIH HHS / United States
AI068524 / AI / NIAID NIH HHS / United States