Disruption of the ECM33 gene in Candida albicans prevents biofilm formation, engineered human oral mucosa tissue damage and gingival cell necrosis/apoptosis.

TitleDisruption of the ECM33 gene in Candida albicans prevents biofilm formation, engineered human oral mucosa tissue damage and gingival cell necrosis/apoptosis.
Publication TypeJournal Article
Year of Publication2012
AuthorsRouabhia M, Semlali A, Chandra J, Mukherjee P, Chmielewski W, Ghannoum MA
JournalMediators Inflamm
Volume2012
Pagination398207
Date Published2012
ISSN1466-1861
KeywordsApoptosis, Biofilms, Candida albicans, Candidiasis, Cells, Cultured, Fungal Proteins, Gingiva, Humans, In Vitro Techniques, Mouth Mucosa, Necrosis
Abstract

In this study we demonstrated that ΔCaecm33 double mutant showed reduced biofilm formation and causes less damage to gingival mucosa tissues. This was confirmed by the reduced level of necrotic cells and Bax/Bcl2 gene expression as apoptotic markers. In contrast, parental and Caecm33 mutant strains decreased basement membrane protein production (laminin 5 and type IV collagen). We thus propose that ECM33 gene/protein represents a novel target for the prevention and treatment of infections caused by Candida.

DOI10.1155/2012/398207
Alternate JournalMediators Inflamm.
PubMed ID22665950
PubMed Central IDPMC3361342
Grant ListR01-DE017486-01A1 / DE / NIDCR NIH HHS / United States
R21-AI74077-01 / AI / NIAID NIH HHS / United States