Exploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses.

TitleExploiting knowledge of immune selection in HIV-1 to detect HIV-specific CD8 T-cell responses.
Publication TypeJournal Article
Year of Publication2010
AuthorsAlmeida C-AM, Roberts SG, Laird R, McKinnon E, Ahmad I, Keane NM, Chopra A, Kadie C, Heckerman D, Mallal S, John M
JournalVaccine
Volume28
Issue37
Pagination6052-7
Date Published2010 Aug 23
ISSN1873-2518
KeywordsCD8-Positive T-Lymphocytes, Consensus Sequence, Epitopes, T-Lymphocyte, Female, HIV Infections, HIV-1, HLA-A Antigens, Humans, Interferon-gamma, Male, nef Gene Products, Human Immunodeficiency Virus, Peptides, Polymorphism, Genetic
Abstract

Since HLA-restricted cytotoxic T-cell responses select specific polymorphisms in HIV-1 sequences and HLA diversity is relatively static in human populations, we investigated the use of peptide epitopes based on sites of HLA-associated adaptation in HIV-1 sequences to stimulate and detect T-cell responses ex vivo. These "HLA-optimised" peptides captured more HIV-1 Nef-specific responses compared with overlapping peptides of a single consensus sequence, in interferon-gamma enzyme linked immunospot assays. Sites of immune selection can reveal more immunogenic epitopes in HLA-diverse populations and offer insights into the nature of HLA-epitope targeting, which could be applied in vaccine design.

DOI10.1016/j.vaccine.2010.06.091
Alternate JournalVaccine
PubMed ID20619380
PubMed Central IDPMC2949439
Grant ListR01 AI060460 / AI / NIAID NIH HHS / United States
R01 AI060460 / AI / NIAID NIH HHS / United States
R01 AI060460-01 / AI / NIAID NIH HHS / United States
R01 AI060460-02 / AI / NIAID NIH HHS / United States
R01 AI060460-03 / AI / NIAID NIH HHS / United States
R01 AI060460-04 / AI / NIAID NIH HHS / United States
R01 AI060460-05 / AI / NIAID NIH HHS / United States