Assessing human immunodeficiency virus type 1 tropism: Comparison of assays using replication-competent virus versus plasma-derived pseudotyped virions.

TitleAssessing human immunodeficiency virus type 1 tropism: Comparison of assays using replication-competent virus versus plasma-derived pseudotyped virions.
Publication TypeJournal Article
Year of Publication2009
AuthorsHosoya N, Su Z, Wilkin T, Gulick RM, Flexner C, Hughes MD, Skolnik PR, Giguel F, Greaves WL, Coakley E, Kuritzkes DR
JournalJ Clin Microbiol
Volume47
Issue8
Pagination2604-6
Date Published2009 Aug
ISSN1098-660X
KeywordsHIV-1, Humans, Plasma, Receptors, Virus, Sensitivity and Specificity, Virology, Virus Internalization, Virus Replication
Abstract

Detection of CXCR4-using human immunodeficiency virus by the Trofile assay was compared to that by assays using virus isolates or replication-competent recombinants. Concordance with the Trofile assay was good, but assays using replicating viruses did not increase substantially the ability to detect the presence of CXCR4-using virus.

DOI10.1128/JCM.00632-09
Alternate JournalJ. Clin. Microbiol.
PubMed ID19494074
PubMed Central IDPMC2725648
Grant ListAI051966 / AI / NIAID NIH HHS / United States
AI055357 / AI / NIAID NIH HHS / United States
AI060354 / AI / NIAID NIH HHS / United States
AI068636 / AI / NIAID NIH HHS / United States
AI069419 / AI / NIAID NIH HHS / United States
AI069472 / AI / NIAID NIH HHS / United States
RR024996 / RR / NCRR NIH HHS / United States
RR16482 / RR / NCRR NIH HHS / United States