Baseline immune phenotypes and CD4+ T lymphocyte responses to antiretroviral therapy in younger versus older HIV-infected individuals.

TitleBaseline immune phenotypes and CD4+ T lymphocyte responses to antiretroviral therapy in younger versus older HIV-infected individuals.
Publication TypeJournal Article
Year of Publication2011
AuthorsHoffman RM, Jamieson BD, Bosch RJ, Currier J, Kitchen CMR, Schmid I, Zhu Y, Bennett K, Mitsuyasu R
JournalJ Clin Immunol
Volume31
Issue5
Pagination873-81
Date Published2011 Oct
ISSN1573-2592
KeywordsAdult, Age Factors, Aged, Antiretroviral Therapy, Highly Active, Biomarkers, Pharmacological, CD4-Positive T-Lymphocytes, Cell Count, Cell Separation, Female, Flow Cytometry, HIV, HIV Infections, Humans, Immunity, Cellular, Male, Middle Aged, Retrospective Studies, Virus Replication
Abstract

OBJECTIVE: The purpose of the study was to determine associations between pre-antiretroviral therapy (ART) senescent CD8+ T lymphocytes and naïve versus non-naive CD8+ and CD4+ T lymphocyte subpopulations and CD4+ responses after initiation of ART in younger versus older individuals.

METHODS: Retrospective analysis of 100 subjects with pre-ART cryopreserved peripheral blood mononuclear cells samples was performed with flow cytometry. Subjects were divided into four groups by age (30-50 years or > 50 years) and 96-week CD4+ response (<100 or >200 cells/mm(3)). All subjects had 96-week viral suppression to <50 copies/mm(3). Regression was utilized to investigate associations between pre-ART CD8+ and CD4+ T cell phenotypes with age and CD4+ response categories.

RESULTS: Individuals <50 years had a lower frequency of senescent CD8+ T lymphocytes of the CD56 + 57+, CD56+, and CD28- phenotypes (95%CI -3.6 to -0.02; 95%CI -4.2 to -0.03; 95%CI -12.5 to -1.4, respectively) and a higher frequency of naïve (CD45RA + CD28+) CD8+ T lymphocytes (95%CI 2.6 to 10.9). Younger age and good CD4+ response were associated with a higher frequency of pre-ART naïve CD4+ T cells (95%CI 2.0 to 16.4 and 95%CI 1.5 to 15.6, respectively).

CONCLUSIONS: Prior to ART, younger HIV-infected individuals have a higher frequency of naïve CD4+ and CD8+ T cells and lower frequency of senescent CD8+ T cell phenotypes.

DOI10.1007/s10875-011-9550-6
Alternate JournalJ. Clin. Immunol.
PubMed ID21643890
PubMed Central IDPMC3194061
Grant ListAG-028748 / AG / NIA NIH HHS / United States
AGO30327 / / PHS HHS / United States
AI-069424 / AI / NIAID NIH HHS / United States
AI-28697 / AI / NIAID NIH HHS / United States
AI-38855 / AI / NIAID NIH HHS / United States
AI-38858 / AI / NIAID NIH HHS / United States
AI-68634 / AI / NIAID NIH HHS / United States
AI-68636 / AI / NIAID NIH HHS / United States
AI028697 / AI / NIAID NIH HHS / United States
CA016042 / CA / NCI NIH HHS / United States
K24 AI056933 / AI / NIAID NIH HHS / United States
P30 AG028748 / AG / NIA NIH HHS / United States
P30 AI028697 / AI / NIAID NIH HHS / United States
P30 CA016042 / CA / NCI NIH HHS / United States
P30-AG028748 / AG / NIA NIH HHS / United States
R01 AG030327 / AG / NIA NIH HHS / United States
U01 AI038855 / AI / NIAID NIH HHS / United States
U01 AI038858 / AI / NIAID NIH HHS / United States
U01 AI068636 / AI / NIAID NIH HHS / United States
U01 AI069424 / AI / NIAID NIH HHS / United States