Blinded, multicenter comparison of methods to detect a drug-resistant mutant of human immunodeficiency virus type 1 at low frequency.

TitleBlinded, multicenter comparison of methods to detect a drug-resistant mutant of human immunodeficiency virus type 1 at low frequency.
Publication TypeJournal Article
Year of Publication2006
AuthorsHalvas EK, Aldrovandi GM, Balfe P, Beck IA, Boltz VF, Coffin JM, Frenkel LM, J Hazelwood D, Johnson VA, Kearney M, Kovacs A, Kuritzkes DR, Metzner KJ, Nissley DV, Nowicki M, Palmer S, Ziermann R, Zhao RY, Jennings CL, Bremer J, Brambilla D, Mellors JW
JournalJ Clin Microbiol
Volume44
Issue7
Pagination2612-4
Date Published2006 Jul
ISSN0095-1137
KeywordsAnti-HIV Agents, Drug Resistance, Viral, HIV Reverse Transcriptase, HIV-1, Humans, Molecular Diagnostic Techniques, Mutation, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Virology
Abstract

We determined the abilities of 10 technologies to detect and quantify a common drug-resistant mutant of human immunodeficiency virus type 1 (lysine to asparagine at codon 103 of the reverse transcriptase) using a blinded test panel containing mutant-wild-type mixtures ranging from 0.01% to 100% mutant. Two technologies, allele-specific reverse transcriptase PCR and a Ty1HRT yeast system, could quantify the mutant down to 0.1 to 0.4%. These technologies should help define the impact of low-frequency drug-resistant mutants on response to antiretroviral therapy.

DOI10.1128/JCM.00449-06
Alternate JournalJ. Clin. Microbiol.
PubMed ID16825395
PubMed Central IDPMC1489464
Grant ListAI-60354 / AI / NIAID NIH HHS / United States
HD 40777 / HD / NICHD NIH HHS / United States
N01-AI-85354 / AI / NIAID NIH HHS / United States
N01-CO-12400 / CO / NCI NIH HHS / United States
P30 AI027767 / AI / NIAID NIH HHS / United States
P30 AI060354 / AI / NIAID NIH HHS / United States
P30AI27767 / AI / NIAID NIH HHS / United States
R01 HD040777 / HD / NICHD NIH HHS / United States
U01 AI038858 / AI / NIAID NIH HHS / United States
U01AI38858 / AI / NIAID NIH HHS / United States