Effect of HIV-1 infection on human DNA yield from saliva.

TitleEffect of HIV-1 infection on human DNA yield from saliva.
Publication TypeJournal Article
Year of Publication2009
AuthorsBasham RJ, Richardson DM, Sutcliffe CB, Haas DW
JournalHIV Clin Trials
Volume10
Issue4
Pagination282-5
Date Published2009 Jul-Aug
ISSN1528-4336
KeywordsAdult, DNA, HIV, HIV Infections, Humans, Middle Aged, Polymerase Chain Reaction, Ribonuclease P, Saliva, Young Adult
Abstract

PURPOSE: Saliva is a good source of DNA for genomic research, and leukocytes are a predominant source of DNA in human saliva. Advanced human immunodeficiency virus (HIV)-type 1 infection disrupts tonsillar architecture and depletes tonsillar lymphocytes. We tested whether HIV-1 infection reduces extracted human DNA yield from saliva.

METHODS: Approximately 2 mL of expectorated saliva was collected from HIV-infected adults during routine primary care clinic visits and from healthy, HIV-negative controls. Human DNA was manually extracted and was specifically quantified by assaying for the RNAse P gene.

RESULTS: Seventy-five individuals were studied, including 25 HIV-infected adults with <200 CD4+ T cells/mm(3) (i.e., acquired immunodeficiency syndrome), 25 with >200 CD4+ T cells/mm3, and 25 HIV-negative controls. Overall DNA yield was 64.7 microg [29.0-139.7 microg] (median [interquartile range]). Yields were comparable among HIV-infected individuals with lower CD4+ T cell counts (74.3 microg [39.4-151.4 microg]), higher CD4+ T cell counts (63.9 microg [29.2-172.1 microg]), and HIV-negative controls (61.4 microg [28.4-123.4 microg]) (p > .05).

CONCLUSION: Infection with HIV-1 does not reduce human DNA yield from saliva. Expectorated saliva should provide sufficient extracted native DNA for genomic studies in HIV-infected individuals.

DOI10.1310/hct1004-282
Alternate JournalHIV Clin Trials
PubMed ID19723616
PubMed Central IDPMC2761620
Grant ListAI068636 / AI / NIAID NIH HHS / United States
AI069439 / AI / NIAID NIH HHS / United States
AI077505 / AI / NIAID NIH HHS / United States
AI54999 / AI / NIAID NIH HHS / United States
KL2 RR024977 / RR / NCRR NIH HHS / United States
P30 AI054999 / AI / NIAID NIH HHS / United States
P30 AI054999-06 / AI / NIAID NIH HHS / United States
R01 AI077505 / AI / NIAID NIH HHS / United States
R01 AI077505-01A1 / AI / NIAID NIH HHS / United States
RR024975 / RR / NCRR NIH HHS / United States
TL1 RR024978 / RR / NCRR NIH HHS / United States
U01 AI068636 / AI / NIAID NIH HHS / United States
U01 AI068636-03 / AI / NIAID NIH HHS / United States
U01 AI069439 / AI / NIAID NIH HHS / United States
U01 AI069439-03 / AI / NIAID NIH HHS / United States
UL1 RR024975 / RR / NCRR NIH HHS / United States
UL1 RR024975-01 / RR / NCRR NIH HHS / United States