HIV-1 viruses detected during episodic blips following interleukin-7 administration are similar to the viruses present before and after interleukin-7 therapy.

TitleHIV-1 viruses detected during episodic blips following interleukin-7 administration are similar to the viruses present before and after interleukin-7 therapy.
Publication TypeJournal Article
Year of Publication2011
AuthorsImamichi H, Degray G, Asmuth DM, Fischl MA, Landay AL, Lederman MM, Sereti I
JournalAIDS
Volume25
Issue2
Pagination159-64
Date Published2011 Jan 14
ISSN1473-5571
KeywordsAntiretroviral Therapy, Highly Active, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Female, Genes, env, HIV Infections, HIV-1, Humans, Interleukin-7, Lymphocyte Activation, Male, Phylogeny, RNA, Viral, Viral Load, Viremia, Virus Latency
Abstract

BACKGROUND: administration of recombinant human interleukin (IL)-7 leads to CD4 and CD8 T-cell expansions in HIV-infected individuals, demonstrating promising capacity for immune reconstitution. However, a proportion of patients treated with recombinant human IL-7 experience transient increases in plasma HIV-RNA ('blips'), possibly reflecting 'purging' of a quiescent reservoir that provides a barrier to viral eradication.

OBJECTIVE: to identify the sources of HIV detected during transient viremic episodes following IL-7 administration, viral quasispecies were analyzed in a total of 281 primary sequences derived from seven patients who experienced the episodic blips following IL-7 therapy.

METHOD: the C2-V3 regions of the HIV-1 env gene were sequenced from HIV-1 RNA in plasma and HIV DNA from peripheral blood mononuclear cells (PBMCs) obtained at baseline (day 0 of recombinant human IL-7 therapy), during the episode of viral blips (day 4), and at a time when levels of plasma HIV-RNA had returned to less than 50 copies/ml (day 28).

RESULTS: the HIV sequences detected during transient viremia following IL-7 administration were closely related to those of the plasma viruses present before and after cytokine administration. All virus quasispecies detected during blips were also present in proviral sequences in PBMCs.

CONCLUSION: the low level viremia induced by IL-7 likely reflects predominantly transient induction or release of virus from a preexisting pool rather than activation of silent quasispecies.

DOI10.1097/QAD.0b013e328340a270
Alternate JournalAIDS
PubMed ID21124203
PubMed Central IDPMC3074174
Grant ListACTG5214 / / PHS HHS / United States
AI 25879 / AI / NIAID NIH HHS / United States
AI 25915 / AI / NIAID NIH HHS / United States
AI 27675 / AI / NIAID NIH HHS / United States
AI 38858 / AI / NIAID NIH HHS / United States
AI 68636 / AI / NIAID NIH HHS / United States
Z99 AI999999 / / Intramural NIH HHS / United States